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primary antibodies against fibroblast activation protein fap  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies against fibroblast activation protein fap
    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
    Primary Antibodies Against Fibroblast Activation Protein Fap, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+fibroblast+activation+protein+fap/bio_rxiv__2025__09__09__675061-112-2-14?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    primary antibodies against fibroblast activation protein fap - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "Bidirectional Crosstalk Between Bladder Cancer Cells and Normal Fibroblasts Drives Phenotypic Reprogramming and Modulates Chemosensitivity"

    Article Title: Bidirectional Crosstalk Between Bladder Cancer Cells and Normal Fibroblasts Drives Phenotypic Reprogramming and Modulates Chemosensitivity

    Journal: bioRxiv

    doi: 10.1101/2025.09.09.675061

    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
    Figure Legend Snippet: (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

    Techniques Used: Flow Cytometry, Cell Culture, Activation Assay, Marker, Staining, Fluorescence, Co-Culture Assay, Expressing



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    primary antibodies against fibroblast activation protein fap  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc primary antibodies against fibroblast activation protein fap
    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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    Image Search Results


    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

    Journal: bioRxiv

    Article Title: Bidirectional Crosstalk Between Bladder Cancer Cells and Normal Fibroblasts Drives Phenotypic Reprogramming and Modulates Chemosensitivity

    doi: 10.1101/2025.09.09.675061

    Figure Lengend Snippet: (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

    Article Snippet: For NF, primary antibodies against fibroblast activation protein (FAP) and α-smooth muscle actin (αSMA) (Cell Signaling Technology) were applied at 1:100 overnight at 4°C.

    Techniques: Flow Cytometry, Cell Culture, Activation Assay, Marker, Staining, Fluorescence, Co-Culture Assay, Expressing